Figure 4.
Changes in the IAA content in spur flower buds and stems at the base of flower buds and immunofluorescence localization of PpyPIN1b in flower buds at 7 days after defoliation. A, Flower bud IAA contents at 0, 5, 10, and 20 days after defoliation in 2018. B, Flower bud IAA contents at 0, 1, 3, 5, 7, and 10 days after defoliation in 2019. C, Stem IAA contents at 0, 3, 7, and 10 days after defoliation. **P < 0.01. D, Left and right panels, respectively, present flower buds from the mock control and defoliated groups at 7 days after defoliation. The fluorescence in the upper images indicates the nuclear and PpyPIN1b fluorescence of flower buds. The fluorescence in the lower magnified images represents the PpyPIN1b protein immunofluorescence localization signal. White arrowheads indicate the cell membrane in which PpyPIN1b was distributed.